COMPARATIVE ANALYSIS OF DAMD, ISSR AND SCOT MOLECULAR MARKERS ON CRYOPRESERVED LUDISIA DISCOLOR AXILLARY BUDS DAMD, ISSR AND SCOT MOLECULAR MARKERS ON CRYOPRESERVED LUDISIA DISCOLOR
Article Sidebar
Main Article Content
Abstract
Ludisia discolor, a jewel orchid is the only species found under its genus (Ludisia). This wild orchid is well known for its striking foliage and its medicinal benefits. Since the population of this valuable species is becoming scarce, therefore protecting this orchid is crucial. Cryopreservation is a practicable long-term germplasm conservation approach. The genetic material is stored at freezing temperature using liquid nitrogen. In this study, V cryo-plate method was developed using L. discolor axillary buds using optimum conditions. For the screening of somaclonal variation, 3 weeks old treated (cryopreserved and non-cryopreserved) explants were utilized in comparison with the stock cultures as a control. The genetic stability was assessed using a total of twenty (20) DAMD, twenty (20) ISSR, and ten (10) SCoT molecular markers. The somaclonal variation detected in cryopreserved explant using DAMD, ISSR, and SCoT molecular markers were 12.5, 4.17, and 10.53% respectively. However, somaclonal variation was also detected in non-cryopreserved explants using DAMD, ISSR, and SCoT at 6.15, 5.77, and 10%, respectively. Hence, SCoT was chosen to be precise than the other two molecular markers (DAMD and ISSR).
Downloads
Article Details
Transfer of Copyrights
- In the event of publication of the manuscript entitled [INSERT MANUSCRIPT TITLE AND REF NO.] in the Malaysian Journal of Science, I hereby transfer copyrights of the manuscript title, abstract and contents to the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) for the full legal term of copyright and any renewals thereof throughout the world in any format, and any media for communication.
Conditions of Publication
- I hereby state that this manuscript to be published is an original work, unpublished in any form prior and I have obtained the necessary permission for the reproduction (or am the owner) of any images, illustrations, tables, charts, figures, maps, photographs and other visual materials of whom the copyrights is owned by a third party.
- This manuscript contains no statements that are contradictory to the relevant local and international laws or that infringes on the rights of others.
- I agree to indemnify the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) in the event of any claims that arise in regards to the above conditions and assume full liability on the published manuscript.
Reviewer’s Responsibilities
- Reviewers must treat the manuscripts received for reviewing process as confidential. It must not be shown or discussed with others without the authorization from the editor of MJS.
- Reviewers assigned must not have conflicts of interest with respect to the original work, the authors of the article or the research funding.
- Reviewers should judge or evaluate the manuscripts objective as possible. The feedback from the reviewers should be express clearly with supporting arguments.
- If the assigned reviewer considers themselves not able to complete the review of the manuscript, they must communicate with the editor, so that the manuscript could be sent to another suitable reviewer.
Copyright: Rights of the Author(s)
- Effective 2007, it will become the policy of the Malaysian Journal of Science (published by the Faculty of Science, University of Malaya) to obtain copyrights of all manuscripts published. This is to facilitate:
- Protection against copyright infringement of the manuscript through copyright breaches or piracy.
- Timely handling of reproduction requests from authorized third parties that are addressed directly to the Faculty of Science, University of Malaya.
- As the author, you may publish the fore-mentioned manuscript, whole or any part thereof, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given. You may produce copies of your manuscript, whole or any part thereof, for teaching purposes or to be provided, on individual basis, to fellow researchers.
- You may include the fore-mentioned manuscript, whole or any part thereof, electronically on a secure network at your affiliated institution, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- You may include the fore-mentioned manuscript, whole or any part thereof, on the World Wide Web, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- In the event that your manuscript, whole or any part thereof, has been requested to be reproduced, for any purpose or in any form approved by the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers), you will be informed. It is requested that any changes to your contact details (especially e-mail addresses) are made known.
Copyright: Role and responsibility of the Author(s)
- In the event of the manuscript to be published in the Malaysian Journal of Science contains materials copyrighted to others prior, it is the responsibility of current author(s) to obtain written permission from the copyright owner or owners.
- This written permission should be submitted with the proof-copy of the manuscript to be published in the Malaysian Journal of Science
References
Adu-Gyamfi R., Wetten A and Marcelino Rodríguez López C.(2016) Effect of cryopreservation and post-cryopreservation somatic embryogenesis on the epigenetic fidelity of cocoa (Theobroma cacao L.). PLoS One, 11(7): 1-13.
Amirah Z J., Elexson N., Grace B and Diyana Z.(2019) Detection of cholera toxin (ctxA and ctxAB) genes in Vibrio cholerae isolated from clinical and environmental samples in Limbang Sarawak by multiplex polymerase chain reaction (PCR). International Postgraduate Symposium in Biotechnology, 2019. 18.
Asnita A H and Norzulaani K.(2006) Direct regeneration and RAPD assessment of male inflorescence derived plants of Musa acuminata cv. Berangan. Asia Pacific Journal of Molecular Biology and Biotechnology, 14(1): 11-17.
Benson E E.(2008) Cryopreservation of phytodiversity: a critical appraisal of theory & practice. Critical reviews in Plant Sciences, 27(3): 141-219.
Collard B C Y and Mackill D J.(2009) Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27(1): 86-93.
Engelmann F.(2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology-Plant, 47(1): 5-16.
Etminan A., Pour-Aboughadareh A., Mohammadi R., Ahmadi-Rad A., Noori A., Mahdavian Z and Moradi Z.(2016) Applicability of start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) markers for genetic diversity analysis in durum wheat genotypes. Biotechnology and Biotechnological Equipment 30(6): 1075–1081.
Gorji A M., Poczai P., Polgar Z and Taller J.(2011) Efficiency of arbitrarily amplified dominant markers (SCoT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato. American Journal of Potato Research, 88(3): 226–237.
Harding K.(2004) Genetic integrity of cryopreserved plant cells: a review. CryoLetters, 25(1): 3-22.
Heath D F., Wei Z., Fowler W K and Nelson V W.(1993) Comparison of spectral radiance calibrations of SBUV-2 satellite ozone monitoring instruments using integrating sphere and flat-plate diffuser techniques. Metrologia, 30(4): 259.
Ibrahim B., Sowemimo A., Van Rooyen A and Van De Venter M.(2012) Anti-inflammatory, analgesic and antioxidant activities of Cyathula prostrata (L.) Blume (Amaranthaceae). Journal of Ethnopharmacol. 141(1): 282–289.
Jain J R., Timsina B., Satyan K B and Manohar S H.(2017) A comparative assessment of morphological and molecular diversity among Sechium edule (Jacq.) Sw. accessions in India. 3 Biotech, 7(2): 1-8.
Kameswara R N.(2004) Plant genetic resources: advancing conservation and use through biotechnology. African Journal of Biotechnology 3(2): 136–145.
Ket N V., Hahn E J., Park S Y., Chakrabarty D and Paek K Y.(2004) Micropropagation of an endangered orchid Anoectochilus formosanus. Biologia Plantarum, 48(3): 339-344.
Lim C L., Jutta M., Kiew R., O'Byrne P and Ong P T.(2013) Conservation status of some orchids in Peninsular Malaysia. Malesian Orchid Journal, 12: 133-146.
Matsumoto T., Yamamoto S I., Fukui K., Rafique T., Engelmann F and Niino T.(2015) Cryopreservation of persimmon shoot tips from dormant buds using the D cryo-plate technique. The Horticulture Journal, MI-043.
Miao N H., Kaneko Y and Sugawara Y.(2005) Ultrastructural implications of pretreatment for successful cryopreservation of Oncidium protocorm-like body. Cry Letters, 26(5): 333- 340.
Mikuła A., Niedzielski M and Rybczyski J J.(2006) The use of TTC reduction assay for assessment of Gentiana spp. cell suspension viability after cryopreservation. Acta Physiologiae Plantarum, 28(4): 315-324.
Moreira A S F P and Isaias R M D S.(2008) Comparative anatomy of the absorption roots of terrestrial and epiphytic orchids. Brazilian Archives of Biology and Technology, 51(1): 83-93.
Nei M and Li W H.(1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences, 76(10): 5269-5273.
Niino, T., Yamamoto, S., Fukui, K., Martínez, C.R., Arizaga M.V., Matsumoto, T., & Engelmann, F.(2013) Dehydration improves cryopreservation of mat rush (Juncus decipiens Nakai) basal stem buds on cryo-plates. CryoLetters 34(6): 549– 560.
Palombi M and Damiano C.(2002) Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Reports, 20(11): 1061-1066.
Poobathy R., Zakaria R., Murugaiyah V and Subramaniam S.(2019) Surface sterilization and micropropagation of Ludisia discolor. Biocatalysis and Agricultural Biotechnology, 22: 101380.
Purayil F T., Robert G A., Gothandam K M., Kurup S S., Subramaniam S and Cheruth A J.(2018) Genetic variability in selected date palm (Phoenix dactylifera L.) cultivars of United Arab Emirates using ISSR and DAMD markers. 3 Biotech, 8(2): 109.
Rawat J M., Rawat B., Mehrotra S., Chandra A and Nautiyal, S.(2013) ISSR and RAPD based evaluation of genetic fidelity and active ingredient analysis of regenerated plants of Picrorhiza kurroa. Acta Physiologiae Plantarum, 35(6): 1797-1805.
Salma M., Fki L., Engelmann-Sylvestre I., Niino T and Engelmann F.(2014) Comparison of droplet-vitrification and D-cryoplate for cryopreservation of date palm (Phoenix dactylifera L.) polyembryonic masses. Scientia Horticulturae, 179: 91-97.
Samarina L S., Malyarovskaya V I., Reim S., Yakushina L G., Koninskaya N G., Klemeshova K V., Shkhalakhov R M., Matskiv A O, Shurkina E S., Slepchenko N A., Ryndin A V and Gabueva, T. Y.(2021) Transferability of ISSR, SCoT and SSR markers for Chrysanthemum× Morifolium Ramat and genetic relationships among commercial Russian cultivars. Plants 2021, 10, 1302.
Seyedimoradi H., Talebi R., Hassani D and Karami F.(2012) Comparative genetic diversity analysis in Iranian local grapevine cultivars using ISSR and DAMD molecular markers. Environmental and Experimental Biology, 10: 125-132.
Suzuki M., Tandon P., Ishikawa M and Toyomasu, T.(2008). Development of a new vitrification solution, VSL, and its application to the cryopreservation of gentian axillary buds. Plant Biotechnology Reports, 2(2): 123-131.
Thakur M., Sharma V and Chauhan A.(2020) Genetic fidelity assessment of long term in vitro shoot cultures and regenerated plants in Japanese plum cvs Santa Rosa and Frontier through RAPD, ISSR and SCoT markers. South African Journal of Botany, 22(1):1-6.
Thammasiri K.(2005) Conservation and sustainable development of orchids in Thailand. In Proceedings of the 17th World Orchid Conference: Sustaining Orchids for the Future 2002. Natural History Publications (Borneo) Sdn Bhd, pp. 211-220.
Tseng C C., Shang H F., Wang L F., Su B., Hsu C C., Kao H Y., Cheng K T.(2006) Antitumor and immune stimulating effects of Anoectochilus formosanus Hayata. Phytomedicine, 13(5): 366-370.
Uchoi A., Malik S K., Choudhary R., Kumar S., Pal D., Rohini M R and Chaudhury R.(2017) Molecular markers in assessing genetic variation of Indian citron (Citrus medica L.) cultivars collected from different parts of India. Indian Journal of Biotechnology, 16(3): 346-356.
Whiters L A.(1985) Cryopreservation of cultured plant cells and protoplasts. Cell culture and somatic cell genetics of plants. 2: 253-316.
Xiong F Q., Tang R H., Chen Z L., Pan L H and Zhuang W J.(2009) SCoT: a novel gene targeted marker technique based on the translation start codon Molecular Plant Breeding. 7(3): 635–638.
Xiong F Q., Jiang J., Zhong R C., Han Z Q., He L Q., Li Z and Tang R H.(2010) Application of SCoT molecular marker in genus Arachis Acta Agronomica Sinica. 36(12): 2055– 2061.
Xiong F., Zhong R., Han Z., Jiang J., He L., Zhuang W and Tang R.(2011) Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes Molecular Biology Reports. 38(5): 3487-3494.
Yamamoto S I., Rafique T., Priyantha W S., Fukui K., Matsumoto T and Niino T.(2011) Development of a cryopreservation procedure using aluminium cryo plates. CryoLetters 32(3): 256-265.
Yin Z F., Zhao B., Bi W L., Chen L and Wang Q C.(2013) Direct shoot regeneration from basal leaf segments of Lilium and assessment of genetic stability in regenerants by ISSR and AFLP markers In Vitro Cellular & Developmental Biology-Plant. 49(3): 333-342.