PHOTOPROTECTIVE EFFECTS OF CARRAGEENANS AGAINST ULTRAVIOLETB-INDUCED EXTRACELLULAR MATRIX (ECM) DAMAGE IN KERATINOCYTES
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Abstract
Carrageenans, the polysaccharide from red seaweeds is widely used in food, medicine and as an excipient in cosmetics and skincare products. Carrageenans have shown prospective photoprotective effect against ultraviolet B (UVB) irradiation on immortalised normal human keratinocyte (HaCaT) cells. The aim of this research was to evaluate the photoprotective effect of iota (ι), kappa (κ)-carrageenans and their combination with vitamin E against UVB-induced extracellular matrix (ECM) damage in HaCaT cells. The study also assessed the superoxide dismutase (SOD) and catalase (CAT) antioxidant enzymes in UVB exposed pre-treated and non-pre-treated cells. The parameters assessed were biological mediators important for the structural integrity of ECM, comprising of epidermal growth factor receptor (EGFR), mitogen-activated protein kinase (MAPK), activating protein- (AP-1) and matrix metalloproteinases (MMPs), liberated by the UVB exposed cells. Enzyme linked immunosorbent assay was used to evaluate the release of these mediators. Carrageenans enhanced the activities of the antioxidant enzymes SOD and CAT which acts as a defence mechanism against oxidative stress. The levels of the biological mediators were also reduced in cells pre-treated with carrageenans, suggesting that the polysaccharide has potential in maintaining the skin’s integrity by reducing the damage to ECM upon UVB exposure. Taken together, the results suggest that carrageenans do possess photoprotective effects against UVB-induced ECM damage and stimulates antioxidant enzymes.
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References
Afaq, F., Mukhtar, H. (2006). Botanical antioxidants in the prevention of photocarcinogenesis and photoaging, Experimental dermatology 15(9): 678-684.
Agatonovic-Kustrin, S., Morton, D. (2013). Cosmeceuticals derived from bioactive substances found in marine algae.
Battie, C., Jitsukawa, S., Bernerd, F., Del Bino, S., Marionnet, C., Verschoore, M. (2014). New insights in photoaging, UVA induced damage and skin types, Experimental dermatology 23, 7-12.
Berneburg, M., Plettenberg, H., Krutmann, J. (2000) Photoaging of human skin, Photodermatology, Photoimmunology & Photomedicine: Review article 16(6): 239-244.
Bowden, G. T. (2004). Prevention of non-melanoma skin cancer by targeting ultraviolet-B-light signalling, Nature Reviews Cancer 4(1): 23-35.
Brenneisen, P., Sies, H., Scharffetter‐Kochanek, K. A. R. I. N. (2002). Ultraviolet‐B irradiation and matrix metalloproteinases: from induction via signaling to initial events, Annals of the New York Academy of Sciences 973(1): 31-43.
Campo, V. L., Kawano, D. F., da Silva Jr, D. B., Carvalho, I. (2009). Carrageenans: Biological properties, chemical modifications and structural analysis–A review, Carbohydrate polymers 77(2): 167-180.
Chen, W., Bowden, G. T. (1999). Activation of p38 MAP kinase and ERK are required for ultraviolet-B induced c-fos gene expression in human keratinocytes, Oncogene 18(52): 7469-7476.
Chung, J. H., Seo, J. Y., Choi, H. R., Lee, M. K., Youn, C. S., Rhie, G. E., Cho K.H., Kim K.H., Park K.C., Eun, H. C. (2001). Modulation of skin collagen metabolism in aged and photoaged human skin in vivo, Journal of Investigative Dermatology 117(5): 1218-1224.
Cian, R. E., Drago, S. R., De Medina, F. S., Martínez-Augustin, O. (2015). Proteins and carbohydrates from red seaweeds: evidence for beneficial effects on gut function and microbiota, Marine drugs 13(8): 5358-5383.
Davis, E. C., Callender, V. D. (2010). Postinflammatory hyperpigmentation: a review of the epidemiology, clinical features, and treatment options in skin of color, The Journal of clinical and aesthetic dermatology 3(7): 20.
Dent, P., Yacoub, A., Fisher, P. B., Hagan, M. P., Grant, S. (2003). MAPK pathways in radiation responses, Oncogene 22(37): 5885-5896.
El-Abaseri, T. B., Putta, S., Hansen, L. A. (2006). Ultraviolet irradiation induces keratinocyte proliferation and epidermal hyperplasia through the activation of the epidermal growth factor receptor, Carcinogenesis 27(2): 225-231.
Fisher, G. J., Datta, S. C., Talwar, H. S., Wang, Z. Q., Varani, J., Kang, S., Voorhees, J. J. (1996). Molecular basis of sun-induced premature skin ageing and retinoid antagonism, Nature, 379(6563): 335-339.
Fisher, G. J., Talwar, H. S., Lin, J., Lin, P., McPhillips, F., Wang, Z., Li X, Wan Y, Kang S, Voorhees, J. J. (1998). Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo, The Journal of clinical investigation 101(6): 1432-1440.
He, G., Cheng, C., Lu, R. (1997). Studies on biological effects of kappa-selenocarrageenan on human breast cancer cell line BCaP-37, Zhonghua yu Fang yi xue za zhi [Chinese Journal of Preventive Medicine] 31(2): 103-106.
Herouy, Y. A. R. E. D. (2001). Matrix metalloproteinases in skin pathology, International journal of molecular medicine 7(1): 3-15.
Hwang, K. A., Yi, B. R., & Choi, K. C. (2011). Molecular mechanisms and in vivo mouse models of skin aging associated with dermal matrix alterations, Laboratory animal research 27(1): 1-8.
Kalitnik, A. A., Barabanova, A. B., Nagorskaya, V. P., Reunov, A. V., Glazunov, V. P., Solov’eva, T. F., Yermak, I. M. (2013). Low molecular weight derivatives of different carrageenan types and their antiviral activity, Journal of applied phycology 25(1): 65-72.
Kang, J., Chen, W., Xia, J., Li, Y., Yang, B., Chen, B., Sun, W., Song, X., Xiang, W., Wang, X. Wang, F., Bi Z., Wan, Y. (2008). Extracellular matrix secreted by senescent fibroblasts induced by UVB promotes cell proliferation in HaCaT cells through PI3K/AKT and ERK signaling pathways. International journal of molecular medicine, 21(6): 777-784.
Kim, M. M., Van Ta, Q., Mendis, E., Rajapakse, N., Jung, W. K., Byun, H. G., Jeon Y.J., Kim, S. K. (2006). Phlorotannins in Ecklonia cava extract inhibit matrix metalloproteinase activity, Life Sciences 79(15): 1436-1443.
Kim, S. J., Ju, J. W., Oh, C. D., Yoon, Y. M., Song, W. K., Kim, J. H., Yoo, Y.J., Bang, O.S., Kang, S.S., Chun, J. S. (2002). ERK-1/2 and p38 kinase oppositely regulate nitric oxide-induced apoptosis of chondrocytes in association with p53, caspase-3, and differentiation status, Journal of Biological Chemistry 277(2): 1332-1339.
Liebel, F., Kaur, S., Ruvolo, E., Kollias, N., Southall, M. D. (2012). Irradiation of skin with visible light induces reactive oxygen species and matrix-degrading enzymes, Journal of Investigative Dermatology 132(7): 1901-1907.
Lin, F. H., Lin, J. Y., Gupta, R. D., Tournas, J. A., Burch, J. A., Selim, M. A., Monteiro-Riviere N.A., Grichnik J.M., Zielinski J., Pinnell, S. R. (2005). Ferulic acid stabilizes a solution of vitamins C and E and doubles its photoprotection of skin, Journal of Investigative Dermatology 125(4): 826-832.
López-Camarillo, C., Aréchaga Ocampo, E., López Casamichana, M., Pérez-Plasencia, C., Álvarez-Sánchez, E., & Marchat, L. A. (2012). Protein kinases and transcription factors activation in response to UV-radiation of skin: implications for carcinogenesis, International journal of molecular sciences 13(1): 142-172.
Mantena, S. K., Katiyar, S. K. (2006). Grape seed proanthocyanidins inhibit UV-radiation-induced oxidative stress and activation of MAPK and NF-κB signaling in human epidermal keratinocytes, Free Radical Biology and Medicine 40(9): 1603-1614.
Meves, A., Stock, S. N., Beyerle, A., Pittelkow, M. R., Peus, D. (2001). H2O2 mediates oxidative stress-induced epidermal growth factor receptor phosphorylation, Toxicology letters 122(3): 205-214.
Moon, H. J., Lee, S. H., Ku, M. J., Yu, B. C., Jeon, M. J., Jeong, S. H., Stonik V.A., Zvyagintseva T.N., Ermakova S.P., Lee, Y. H. (2009). Fucoidan inhibits UVB-induced MMP-1 promoter expression and down regulation of type I procollagen synthesis in human skin fibroblasts, European Journal of Dermatology 19(2): 129-134.
Moon, H. J., Lee, S. R., Shim, S. N., Jeong, S. H., Stonik, V. A., Rasskazov, V. A., Zvyagintseva, T., Lee, Y. H. (2008). Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts, Biological and Pharmaceutical Bulletin 31(2): 284-289.
Munshi, A., Ramesh, R. (2013). Mitogen-activated protein kinases and their role in radiation response, Genes & cancer 4(9-10): 401-408.
Narang, H., Bhat, N., Gupta, S. K., Santra, S., Choudhary, R. K., Kailash, S., Krishna, M. (2009). Differential activation of mitogen-activated protein kinases following high and low LET radiation in murine macrophage cell line, Molecular and cellular biochemistry 324(1): 85-91.
Necas, J., Bartosikova, L. (2013). Carrageenan: a review, Veterinarni medicina 58(4).
Ouwens, D. M., de Mesquita, D. S. G., Dekker, J., & Maassen, J. A. (2001). Hyperosmotic stress activates the insulin receptor in CHO cells, Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1540(2): 97-106.
Pandel, R., Poljšak, B., Godic, A., Dahmane, R. (2013). Skin photoaging and the role of antioxidants in its prevention, International Scholarly Research Notices 2013.
Quan, T., Qin, Z., Xia, W., Shao, Y., Voorhees, J. J., Fisher, G. J. (2009, August). Matrix-degrading metalloproteinases in photoaging, In Journal of Investigative Dermatology Symposium Proceedings (Vol. 14, No. 1, pp. 20-24). Elsevier.
Rabe, J. H., Mamelak, A. J., McElgunn, P. J., Morison, W. L., Sauder, D. N. (2006) Photoaging: mechanisms and repair Journal of the American Academy of Dermatology 55(1): 1-19.
Ren, S. W., Li, J., Wang, W., & Guan, H. S. (2010). Protective effects of κ-ca3000+ CP against ultraviolet-induced damage in HaCaT and MEF cells, Journal of Photochemistry and Photobiology B: Biology 101(1): 22-30.
Rezvani, H. R., Mazurier, F., Cario-André, M., Pain, C., Ged, C., Taïeb, A., de Verneuil, H. (2006). Protective effects of catalase overexpression on UVB-induced apoptosis in normal human keratinocytes, Journal of Biological Chemistry 281(26): 17999-18007.
Sachs, D., Helfrich, Y., Voorhees, J. (2009). Molecular mechanisms in skin aging, COSMETIC DERMATOLOGY-CEDAR KNOLLS- 22(6): 291-298.
Saeed, A. K., & Salmo, N. (2012). Epidermal growth factor receptor expression in mice skin upon ultraviolet B exposure-Seborrheic Keratosis as a coincidental and unique finding, Advanced biomedical research, 1.
Sasaki, H., Akamatsu, H., Horio, T. (1997). Effects of a single exposure to UVB radiation on the activities and protein levels of copper‐zinc and manganese superoxide dismutase in cultured human keratinocytes, Photochemistry and photobiology 65(4): 707-713.
Sokolova, E. V., Barabanova, A. O., Homenko, V. A., Solov'eva, T. F., Bogdanovich, R. N., Yermak, I. M. (2011). In vitro and ex vivo studies of antioxidant activity of carrageenans, sulfated polysaccharides from red algae. Bulletin of experimental biology and medicine 150(4): 426.
Sun, Z., Park, S. Y., Hwang, E., Zhang, M., Seo, S. A., Lin, P., Yi, T. H. (2017). Thymus vulgaris alleviates UVB irradiation induced skin damage via inhibition of MAPK/AP‐1 and activation of Nrf2‐ARE antioxidant system, Journal of cellular and molecular medicine 21(2): 336-348.
Taher, M. M., Hershey, C. M., Oakley, J. D., & Valerie, K. (2000). Role of the p38 and MEK‐½/p42/44 MAP Kinase Pathways in the Differential Activation of Human Immunodeficiency Virus Gene Expression by Ultraviolet and Ionizing Radiation, Photochemistry and photobiology 71(4): 455-459.
Thevanayagam, H., Mohamed, S. M., Chu, W. L. (2014). Assessment of UVB-photoprotective and antioxidative activities of carrageenan in keratinocytes, Journal of applied phycology 26(4): 1813-1821.
Wen, K. C., Fan, P. C., Tsai, S. Y., Shih, I., Chiang, H. M. (2012). Ixora parviflora protects against UVB-induced photoaging by inhibiting the expression of MMPs, MAP kinases, and COX-2 and by promoting type I procollagen synthesis, Evidence-Based Complementary and Alternative Medicine, 2012.
Yasui, H., Sakurai, H. (2003). Age‐dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light, Experimental dermatology 12(5): 655-661.
Yuan, H., Song, J., Zhang, W., Li, X., Li, N., Gao, X. (2006). Antioxidant activity and cytoprotective effect of κ-carrageenan oligosaccharides and their different derivatives, Bioorganic & Medicinal Chemistry Letters 16(5): 1329-1334.
Zhang, Z., Wang, F., Wang, X., Liu, X., Hou, Y., Zhang, Q. (2010). Extraction of the polysaccharides from five algae and their potential antioxidant activity in vitro, Carbohydrate Polymers 82(1): 118-121.
Zussman, J., Ahdout, J., Kim, J. (2010). Vitamins and photoaging: Do scientific data support their use?. Journal of the American Academy of Dermatology 63(3): 507-525.